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This assay was applied to both cultured cells and frozen tissue sections, with tissue sections counterstained with Nuclear Fast Red for better visualization

Hence, we chose a prophylactic low dose (1 M) of QCT to counteract Erastin-induced endothelial dysfunction

Limitations of this approach, however, include the lack of quantitative analysis and of a structured assessment of the quality of studies and certainty of evidence, which were beyond the scope of this descriptive work

References: (1) Thymosin beta4 accelerates wound healing

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